The bus was carrying 23 players and coaches with the Seton Hill University women's lacrosse team when it crashed on the Pennsylvania Turnpike on the way to a game at Millersville University.
The accident killed 30-year-old women's lacrosse coach Kristina Quigley, her unborn child and the bus driver, 61-year-old Johnstown resident Anthony Guaetta.
Cumberland County Coroner Charles Hall said Monday that toxicology results showed Guaetta didn't have any drugs or alcohol in his system. They also showed he didn't have a medical emergency.
The cause of the crash hasn't been determined.